Glarm’s Weblog

BioMinds Undergraduate Investigator

Isolation and Characterization of Puerto Rican Soils Bioprospects Resistant to Metals Marzo 2, 2009

Archivado en: General — Astrobiología una nueva visión científica @ 9:11 am

Abstract

Astrobiology is a novel interdisciplinary science that studies the origin, evolution and distribution of life on Earth and in the Universe. One of the NASA’s program priorities involves the analysis of microbes as a source to understand the response of organisms to conditions in space or other planets.  Possible bioprospects are microorganisms that live or survive extreme environments, such as high concentrations of metals. These bioprospects could represent an important source of information for NASA about life in environments with similar extreme conditions.  The main focus of this research is to isolate and characterize microorganisms that can tolerate high concentrations of metals to further understand their resistant mechanism.  Environmental samples were processed by serial dilutions and spreaded on a media supplemented with different concentrations of copper. Then, the metal resistant microbes (MR-Microbes) were initially characterized microbiologically.  A total of eight bioprospects were obtained from different environmental samples.  The macroscopic characterization shows that most of the colonies have a regular shape, but only two shows an irregular shape.  Also the colonies showed pigmentation which range from white to blue/green.  The isolates showed two types of texture cottony and powdery.  Microscopically, some of the bioprospects presented asexual reproduction structures such as vesicle, phialides and conidias that are associated with the genus Aspergillus sp.  Ongoing research is being performed to start analyzing the molecular physiology of the sample.

 

 

Progreso Obtenido Marzo 2, 2009

Archivado en: General — Astrobiología una nueva visión científica @ 9:09 am

El progreso que he obtenido en m investigación se puede decir que es de aproxidamente de un mas de un 50% ya que aun me falta realizar algunas pruebas para poder completar la caracterización de los bioprospectos.  Me hubiera gustado haber podido tener más resultados pero me he tenido que enfrentar con varias circunstancias que le pueden haber ocurrido a cualquiera de mis compañeros, como son el tiempo en que tardan mis hongos en crecer que son de aproximadamente 7 días, la contaminación que han sufrido mis microorganismos en el tiempo de incubación, lo que me ha llevado a volver a inocularlos en platos petris nuevos y por ultimo el factor tiempo ya que a veces se me hace un poco difícil el poder organizar todas mis tareas académicas mas la investigación sin dejar de descuidar ninguna de ellas.